Proteome analysis reveals an energy-dependent central process for Populus×canadensis seed germination.
Identifieur interne : 001237 ( Main/Exploration ); précédent : 001236; suivant : 001238Proteome analysis reveals an energy-dependent central process for Populus×canadensis seed germination.
Auteurs : Hong Zhang [République populaire de Chine] ; Ke-Xin Zhou [République populaire de Chine] ; Wei-Qing Wang [République populaire de Chine] ; Shu-Jun Liu [République populaire de Chine] ; Song-Quan Song [République populaire de Chine]Source :
- Journal of plant physiology [ 1618-1328 ] ; 2017.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : Acide abscissique, Cycloheximide, Graines, Populus, Protéines végétales, Protéome.
- physiologie : Germination, Graines, Populus.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Abscisic Acid, Cycloheximide, Plant Proteins, Proteome.
- metabolism : Populus, Seeds.
- physiology : Germination, Populus, Seeds.
Abstract
Poplar (Populus×canadensis) seeds rapidly germinated in darkness at 10, 15, and 20°C and reached 50% seed germination after about 22, 4.5, and 3.5h, respectively. Germination of poplar seeds was markedly inhibited by abscisic acid (ABA) at 50μM and cycloheximide (CHX) at 100μM, and these inhibitive roles were temperature-dependent. In the present study, mature poplar seeds were used to investigate the differentially changed proteome of seeds germinating in water, ABA, and CHX. A total of 130 protein spots showed a significant change (1.5-fold increase/decrease, P<0.05) in abundance, and 101 protein spots were successfully identified. Most of the proteins were associated with cell defense and rescue (21%), storage proteins (21%), protein synthesis and destination (20%), metabolism (16%), and energy (14%). The germination of poplar seeds is closely related with the increase in those proteins involved in amino acid and lipid metabolism, the tricarboxylic acid cycle and pentose phosphate pathway, protein synthesis and destination, cell defense and rescue, and degradation of storage proteins. ABA and CHX inhibit the germination of poplar seeds by decreasing the protein abundance associated with protein proteolysis, protein folding, and storage proteins. We conclude that poplar seed germination is an energy-dependent active process, and is accompanied by increasing amino acid activation, protein synthesis and destination, as well as cell defense and rescue, and degradation of storage proteins.
DOI: 10.1016/j.jplph.2017.03.008
PubMed: 28384531
Affiliations:
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Electronic address: sqsong@ibcas.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">Journal of plant physiology</title><idno type="eISSN">1618-1328</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Abscisic Acid (metabolism)</term><term>Cycloheximide (metabolism)</term><term>Germination (physiology)</term><term>Plant Proteins (metabolism)</term><term>Populus (metabolism)</term><term>Populus (physiology)</term><term>Proteome (metabolism)</term><term>Seeds (metabolism)</term><term>Seeds (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acide abscissique (métabolisme)</term><term>Cycloheximide (métabolisme)</term><term>Germination (physiologie)</term><term>Graines (métabolisme)</term><term>Graines (physiologie)</term><term>Populus (métabolisme)</term><term>Populus (physiologie)</term><term>Protéines végétales (métabolisme)</term><term>Protéome (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Abscisic Acid</term><term>Cycloheximide</term><term>Plant Proteins</term><term>Proteome</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term><term>Seeds</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acide abscissique</term><term>Cycloheximide</term><term>Graines</term><term>Populus</term><term>Protéines végétales</term><term>Protéome</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Germination</term><term>Graines</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Germination</term><term>Populus</term><term>Seeds</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Poplar (Populus×canadensis) seeds rapidly germinated in darkness at 10, 15, and 20°C and reached 50% seed germination after about 22, 4.5, and 3.5h, respectively. Germination of poplar seeds was markedly inhibited by abscisic acid (ABA) at 50μM and cycloheximide (CHX) at 100μM, and these inhibitive roles were temperature-dependent. In the present study, mature poplar seeds were used to investigate the differentially changed proteome of seeds germinating in water, ABA, and CHX. A total of 130 protein spots showed a significant change (1.5-fold increase/decrease, P<0.05) in abundance, and 101 protein spots were successfully identified. Most of the proteins were associated with cell defense and rescue (21%), storage proteins (21%), protein synthesis and destination (20%), metabolism (16%), and energy (14%). The germination of poplar seeds is closely related with the increase in those proteins involved in amino acid and lipid metabolism, the tricarboxylic acid cycle and pentose phosphate pathway, protein synthesis and destination, cell defense and rescue, and degradation of storage proteins. ABA and CHX inhibit the germination of poplar seeds by decreasing the protein abundance associated with protein proteolysis, protein folding, and storage proteins. We conclude that poplar seed germination is an energy-dependent active process, and is accompanied by increasing amino acid activation, protein synthesis and destination, as well as cell defense and rescue, and degradation of storage proteins.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28384531</PMID><DateCompleted><Year>2017</Year><Month>11</Month><Day>27</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1618-1328</ISSN><JournalIssue CitedMedium="Internet"><Volume>213</Volume><PubDate><Year>2017</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Journal of plant physiology</Title><ISOAbbreviation>J Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Proteome analysis reveals an energy-dependent central process for Populus×canadensis seed germination.</ArticleTitle><Pagination><MedlinePgn>134-147</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0176-1617(17)30073-1</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jplph.2017.03.008</ELocationID><Abstract><AbstractText>Poplar (Populus×canadensis) seeds rapidly germinated in darkness at 10, 15, and 20°C and reached 50% seed germination after about 22, 4.5, and 3.5h, respectively. Germination of poplar seeds was markedly inhibited by abscisic acid (ABA) at 50μM and cycloheximide (CHX) at 100μM, and these inhibitive roles were temperature-dependent. In the present study, mature poplar seeds were used to investigate the differentially changed proteome of seeds germinating in water, ABA, and CHX. A total of 130 protein spots showed a significant change (1.5-fold increase/decrease, P<0.05) in abundance, and 101 protein spots were successfully identified. Most of the proteins were associated with cell defense and rescue (21%), storage proteins (21%), protein synthesis and destination (20%), metabolism (16%), and energy (14%). The germination of poplar seeds is closely related with the increase in those proteins involved in amino acid and lipid metabolism, the tricarboxylic acid cycle and pentose phosphate pathway, protein synthesis and destination, cell defense and rescue, and degradation of storage proteins. ABA and CHX inhibit the germination of poplar seeds by decreasing the protein abundance associated with protein proteolysis, protein folding, and storage proteins. We conclude that poplar seed germination is an energy-dependent active process, and is accompanied by increasing amino acid activation, protein synthesis and destination, as well as cell defense and rescue, and degradation of storage proteins.</AbstractText><CopyrightInformation>Copyright © 2017 Elsevier GmbH. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Hong</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Ke-Xin</ForeName><Initials>KX</Initials><AffiliationInfo><Affiliation>Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Wei-Qing</ForeName><Initials>WQ</Initials><AffiliationInfo><Affiliation>Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Shu-Jun</ForeName><Initials>SJ</Initials><AffiliationInfo><Affiliation>Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Song-Quan</ForeName><Initials>SQ</Initials><AffiliationInfo><Affiliation>Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China. Electronic address: sqsong@ibcas.ac.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>03</Month><Day>18</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>J Plant Physiol</MedlineTA><NlmUniqueID>9882059</NlmUniqueID><ISSNLinking>0176-1617</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020543">Proteome</NameOfSubstance></Chemical><Chemical><RegistryNumber>72S9A8J5GW</RegistryNumber><NameOfSubstance UI="D000040">Abscisic Acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>98600C0908</RegistryNumber><NameOfSubstance UI="D003513">Cycloheximide</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000040" MajorTopicYN="N">Abscisic Acid</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003513" MajorTopicYN="N">Cycloheximide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018525" MajorTopicYN="N">Germination</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020543" MajorTopicYN="N">Proteome</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012639" MajorTopicYN="N">Seeds</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">ABA</Keyword><Keyword MajorTopicYN="N">Cycloheximide</Keyword><Keyword MajorTopicYN="N">Germination</Keyword><Keyword MajorTopicYN="N">Poplar seed</Keyword><Keyword MajorTopicYN="N">Proteome</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>01</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2017</Year><Month>03</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>03</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>4</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>11</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>4</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28384531</ArticleId><ArticleId IdType="pii">S0176-1617(17)30073-1</ArticleId><ArticleId IdType="doi">10.1016/j.jplph.2017.03.008</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhang, Hong" sort="Zhang, Hong" uniqKey="Zhang H" first="Hong" last="Zhang">Hong Zhang</name></noRegion><name sortKey="Liu, Shu Jun" sort="Liu, Shu Jun" uniqKey="Liu S" first="Shu-Jun" last="Liu">Shu-Jun Liu</name><name sortKey="Song, Song Quan" sort="Song, Song Quan" uniqKey="Song S" first="Song-Quan" last="Song">Song-Quan Song</name><name sortKey="Wang, Wei Qing" sort="Wang, Wei Qing" uniqKey="Wang W" first="Wei-Qing" last="Wang">Wei-Qing Wang</name><name sortKey="Zhou, Ke Xin" sort="Zhou, Ke Xin" uniqKey="Zhou K" first="Ke-Xin" last="Zhou">Ke-Xin Zhou</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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